Complete amino acid sequence of luffin-a, a ribosome-inactivating protein from the seeds of Luffa cylindrica.
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منابع مشابه
Complete amino acid sequence of luffin-b, a ribosome-inactivating protein from sponge gourd (Luffa cylindrica) seeds.
The complete amino acid sequence of luffin-b has been determined. All the twenty-seven tryptic peptides were isolated by reverse-phase HPLC from the tryptic digests of intact luffin-b and one of its CNBr fragments (CB4), and sequenced using the DABITC/PITC double coupling method. The overlap of these peptides was achieved by analyzing the CNBr fragments and their chymotryptic peptides. Luffin-b...
متن کاملIsolation and partial characterization of three protein-synthesis inhibitory proteins from the seeds of Luffa cylindrica.
Three new proteins which inhibit protein synthesis in rabbit reticulocyte lysates were isolated from an extract of sponge gourd (Luffa cylindrica) seeds by chromatography on a AF-Blue Toyopearl column followed by FPLC with a Mono S column. These three protein-synthesis inhibitory proteins (PSIs) have molecular masses of 19 kDa, 15 kDa, and 9 kDa, and were designated 19K-PSI, 15K-PSI, and 9K-PSI...
متن کاملOptimization of EnBase Fed-Batch Cultivation to Improve Soluble Fraction Ratio of α-Luffin Ribosome Inactivating Protein
Background: The increase of the protein expression via ribosomal manipulation is one of the suggested cellular mechanisms involved in EnBase fed-batch mode of cultivation. However, this system has not been implemented for cytotoxic proteins.Objectives: Here, the expression pattern of α-Luffin, a ribosome inactivation protein (RIP) with an innate toxicity,...
متن کاملThe complete amino acid sequence of chitinase-a from the seeds of rye (Secale cereal).
The complete amino acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleaved with cyanogen bromide and the resulting three fragments, CB1, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CB1 were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin of reduced S-carboxymethylated ...
متن کاملThe Complete Amino Acid Sequence of Chitinase - a from the Seeds of Rye ( Slecale cereat
The complete amine acid sequence of rye seed chitinase-a (RSC-a) has been analyzed. RSC-a was cleayed with cyanogen bromide and the resulting three fragments, CBI, CB2, and CB3, were separated by gel filtration. The amino acids of the N-terminal fragment CBI were sequenced by analyzing the peptides produced by digestion with trypsin, lysylendopeptidase, or pepsin ef reduced SLcarboxymethylated ...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.1343